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Induced Pluripotent Stem Cells
Colonies of induced pluripotentstem cells. Blue shows the DNA (nucleus) of all the cells, including the background cells used for feeding stem cells. Green staining shows only stem cells.
Induced pluripotent stem cells generated at the CSIR differentiate into beating cardiomyocytes (heart cells).
Click on the image to the left to watch the video.
Applet for Microscope Resolution Calculations
A 3D real-time PALM/STORM image processing plugin for ImageJ: QuickPALM website
QuickPALM is a set of programs to aid in the acquisition and image analysis of data in “photoactivated light microscopy” (PALM) and “stochastic optical light microscopy” (STORM).
World Practical Course on Microscopy:
from single molecules to animals
This course will be aimed at advanced Ph.D. students and post-docs who would like to learn new and emerging techniques in infection biology and light microscopy.
The major objective of this practical course is to introduce standard, super-resolution, high throughput microscopy and imaging technologies to identify and characterize key molecules involved in infection. Ideally this will include screening a small gene family (via siRNA). Special attention will be paid to how cell based assays are developed to first identify the phenomenon, and then used to give robust and unambiguous results when screened. Students will get hands-on use of scanning confocal, nipkow disk confocal and super-resolution methods and learn the basics of image processing.
Course Organizers:
Musa Mhlanga, Jost Enninga, Freddy Frischknecht
To learn more about this course as well as register to participate, please visit the course website.
